Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3

Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rat isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies spe...

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Bibliographic Details
Published inHybridoma Vol. 8; no. 4; p. 475
Main Authors Vos, R M, Lutkeschipholt, I J, Boonekamp, P M, Schots, A, Pomp, H, Van Bladeren, P J, Koeman, J H
Format Journal Article
LanguageEnglish
Published United States 01.08.1989
Subjects
Animals
Antibodies, Monoclonal
Antibody Specificity
Cross Reactions
Glutathione Transferase - immunology
Hybridomas - immunology
Isoenzymes - immunology
Mice
Mice, Inbred Strains
Rats
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Summary:Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rat isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3. Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.
ISSN:0272-457X
DOI:10.1089/hyb.1989.8.475