Single-cell adhesion probed in-situ using optical tweezers: A case study with Saccharomyces cerevisiae

A facile method of using optical trapping to measure cell adhesion forces is presented and applied to the adhesion of Saccharomyces cerevisiae on glass, in contact with solutions of different compositions. Trapping yeast cells with optical tweezers (OT) is not perturbed by cell wall deformation or c...

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Bibliographic Details
Published inJournal of applied physics Vol. 111; no. 11; pp. 114701 - 114701-13
Main Authors Castelain, Mickaël, Rouxhet, Paul G., Pignon, Frédéric, Magnin, Albert, Piau, Jean-Michel
Format Journal Article
LanguageEnglish
Published American Institute of Physics 01.06.2012
Subjects
Life Sciences
Other
surface hydrophobicity
red-blood-cells
stainless-steel
flow induced detachment
escherichia-coli
biofilm formation
atomic-force microscopy
azospirillum-brasilense
yeast-cells
bacterial adhesion
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Summary:A facile method of using optical trapping to measure cell adhesion forces is presented and applied to the adhesion of Saccharomyces cerevisiae on glass, in contact with solutions of different compositions. Trapping yeast cells with optical tweezers (OT) is not perturbed by cell wall deformation or cell deviation from a spherical shape. The trapping force calibration requires correction not only for the hydrodynamic effect of the neighboring wall but also for spherical aberrations affecting the focal volume and the trap stiffness. Yeast cells trapped for up to 5h were still able to undergo budding but showed an increase of doubling time. The proportion of adhering cells showed the expected variation according to the solution composition. The detachment force varied in the same way. This observation and the fact that the detachment stress was exerted parallel to the substrate surface point to the role of interactions involving solvated macromolecules. Both the proportion of adhering cells and the removal force showed a distribution which, in our experimental conditions, must be attributed to a heterogeneity of surface properties at the cell level or at the subcellular scale. As compared with magnetic tweezers, atomic force microscopy, and more conventional ways of studying cell adhesion (shear-flow cells), OT present several advantages that are emphasized in this paper.
ISSN:0021-8979
1089-7550
1089-7550
DOI:10.1063/1.4723566