Distribution of Antibiotic Resistance and Antibiotic Resistant Genes in Campylobacter jejuni Isolated from Poultry in North West of Pakistan

ABSTRACT Campylobacter species are one of the most important food borne zoonotic pathogens. A total of 1260 poultry meat samples were collected from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni w...

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Published inPakistan journal of zoology Vol. 53; no. 1; p. 79
Main Authors Khan, Sher Bahadar, Khan, Mumtaz Ali, Khan, Hameed Ullah, Khan, Sher Ali, Fahad, Shah, Khan, Faheem Ahmad, Ahmad, Irshad, Nawaz, Nighat, Bibi, Sidra, Muneeb, Muhammad
Format Journal Article
LanguageEnglish
Published Lahore Knowledge Bylanes 28.02.2021
AsiaNet Pakistan (Pvt) Ltd
Subjects
AMP
Antibiotic resistance
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Campylobacter
Campylobacter jejuni
Causes of
Drug resistance
Drug resistance in microorganisms
Enrichment media
Food contamination & poisoning
Genes
Genetic aspects
Health aspects
Laboratories
Meat
Morphology
Multidrug resistance
Pathogens
Poultry
Public health
Zoonoses
Pakistan
United States > US
Peshawar Pakistan
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Summary:ABSTRACT Campylobacter species are one of the most important food borne zoonotic pathogens. A total of 1260 poultry meat samples were collected from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX (27%), CIP (25%), LFX (13%) and AZM (11%) while the least resistance was found against GAT (8%) and CRO (9%). 90% isolates were found to have multiple drug resistance. As for as antibiotic resistant genes are concerned, the highest ARG was blaTEM (93%) followed by tetA (82%), sul2 (75%), blaSHV (72%), tetC (71%), strA/strB (50%), sul1 (49%), blaCMY2 and aadA (44%) while the least resistant gene was aadb (9%) followed by sul3 (21%) and aac(3)IV (37%). About 92% isolates were found to have multiple drug resistance genes which is a matter of great concern from human public health perspective.
ISSN:0030-9923
DOI:10.17582/JOURNAL.PJZ/20190828140843