RT-PCR Suggests Human Skeletal Muscle Origin of Alveolar Soft-Part Sarcoma

• Published in: Oncology Vol. 58; no. 4; pp. 319 - 323
• Main Authors: Nakano, Hirotaka, Tateishi, Akio, Imamura, Tetsuo, Miki, Hiroshi, Ohno, Tohgo, Moue, Toshiko, Sekiguchi, Mayumi, Unno, Kazutoshi, Abe, Satoshi, Matsushita, Takashi, Katoh, Yoshiharu, Itoh, Tatsuo
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2000
• Subjects: Actins - genetics; DNA Primers; DNA, Complementary - analysis; DNA-Binding Proteins - genetics; Forkhead Box Protein O1; Forkhead Transcription Factors; Homeodomain Proteins; Humans; Laboratory/Clinical Translational Research; Muscle Proteins - genetics; Muscle, Skeletal; MyoD Protein - genetics; Myogenin - genetics; Nerve Tissue Proteins - genetics; Paired Box Transcription Factors; PAX3 Transcription Factor; PAX7 Transcription Factor; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - analysis; Sarcoma, Alveolar Soft Part - genetics; Sarcoma, Alveolar Soft Part - pathology; Transcription Factors - genetics; Histogenesis; Complementary DNA; Alveolar soft part sarcoma; Reverse transcription polymerase chain reaction; Skeletal-muscle-related mRNA
• ISSN: 0030-2414; 1423-0232
• DOI: 10.1159/000012119

In 1952, Christopherson et al. proposed that alveolar soft-part sarcoma (ASPS) was a distinct entity with unique clinical and pathological features. Since their report, its histogenesis has not been determined. In order to clarify the histogenesis of ASPS, a study using reverse transcription polymerase chain reaction using cDNAs from MyoD1 and myogenin, and the actin filament from human-skeletal-muscle-related mRNAs has been performed in 5 cases of ASPS. The expression of MyoD1 and myogenin was determined in 5 and 2 cases of the 5 cases, respectively. Moreover, expression of the many mRNAs from the actin filament of skeletal muscle was also found in ASPS. According to these findings, it is now postulated that ASPS is of skeletal muscle origin.