Presence of multiple human papillomavirus types in cervical samples from HIV-infected women

• Published in: Gynecologic oncology Vol. 92; no. 1; pp. 225 - 231
• Main Authors: Levi, José Eduardo, Fernandes, Silvana, Tateno, Adriana Fumie, Motta, Eduardo, Lima, Liliam Pereira, Eluf-Neto, José, Pannuti, Cláudio Sérgio
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2004
• Subjects: Adolescent; Adult; Aged; Cervical intraepithelial neoplasia; Cervical Intraepithelial Neoplasia - immunology; Cervical Intraepithelial Neoplasia - virology; Female; Genotype; Genotyping; HIV Infections - complications; HIV Infections - immunology; HIV Infections - virology; HPV; Humans; Middle Aged; Multiple infections; Papillomaviridae - genetics; Papillomavirus Infections - complications; Papillomavirus Infections - immunology; Papillomavirus Infections - virology; PCR; Polymerase Chain Reaction; Tumor Virus Infections - complications; Tumor Virus Infections - immunology; Tumor Virus Infections - virology; Uterine Cervical Neoplasms - immunology; Uterine Cervical Neoplasms - virology; Viral Load; Multiple infections; Genotyping; Viral load; Cervical intraepithelial neoplasia; PCR; HPV
• ISSN: 0090-8258; 1095-6859
• DOI: 10.1016/j.ygyno.2003.10.004

Objectives. The aim of this study was to detect and identify human papillomavirus (HPV) genotypes on a population of women infected by the human immunodeficiency virus (HIV) and to investigate the role of multiple infections on cervical dysplasia. Methods. Two hundred and fifty-five HIV-infected women were enrolled on a study to evaluate the prevalence of HPV and cervical intra-epithelial neoplasia (CIN). A group of HIV-negative women with confirmed CIN diagnosis was included for comparison. A polymerase chain reaction (PCR)-reverse hybridization method was applied to detect and precisely identify HPV types, specifically multiple infections. Results. On HIV patients, an altered Pap smear confirmed by biopsy was observed on 45 (18%); HPV-DNA prevalence was 87% (223/255), with 45% (116/255) infected by more than two types. In contrast, HPV-DNA was detected in all 36 women of the control group but only 3 were infected by more than two types. Cervical dysplasia was associated with low CD4 counts and elevated high-risk HPV viral load. However, the presence of multiple HPV types did not correlate with the degree of immune suppression or the presence of cervical lesions. Conclusions. Infection with multiple HPV types is a rather frequent finding on Brazilian HIV-infected women. On this population, concomitant infection with three or more HPV types does not seem to confer an additional risk of cervical dysplasia in comparison to single/double infections, nor to be related to more severe immunesuppresion.