Determination of manidipine enantiomers in human serum using chiral chromatography and column-switching liquid chromatography

• Published in: Journal of chromatography Vol. 575; no. 1; pp. 123 - 129
• Main Authors: Yamaguchi, Masashi, Yamashita, Kenji, Aoki, Isamu, Tabata, Tetsuro, Hirai, Shin-Ichiro, Yashiki, Takatsuka
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 13.03.1992; Elsevier
• Subjects: Administration, Oral; Antihypertensive agents; Biological and medical sciences; Calcium Channel Blockers - administration & dosage; Calcium Channel Blockers - blood; Cardiovascular system; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid - methods; Dihydropyridines - administration & dosage; Dihydropyridines - blood; Humans; Medical sciences; Pharmacology. Drug treatments; Stereoisomerism; Stereoselectivity; Antihypertensive agent; Measurement method; Enantiomer; Calcium antagonist
• ISSN: 0378-4347
• DOI: 10.1016/0378-4347(92)80512-O

A stereoselective and highly sensitive method using chiral chromatography and successive column-switching liquid chromatography is described for the determination of manidipine enantiomers in human serum. A human serum sample obtained after ingestion of manidipine was extracted twice with a mixture of n-hexane—diethyl ether under alkaline conditions. The enantiomers in the extract were separated on a chiral stationary phase column (Chiralcel OJ), and the effluents containing the respective enantiomers were collected. Each fraction was then analysed by column-switching liquid chromatography. The proposed stereoselective method offered high sensitivity: detection limits for both isomers were 0.2 ng/ml in human serum, both at a signal-to-noise ratio of 3. The method is suitable for the pharmacokinetic studies of manidipine enantiomers.