Bioelectrophoresis: a rapid procedure for the identification of antimicrobial agents

Sensitivity discs were placed on 1% Noble agar for electrophoresis in a 20 mM phosphate-buffered, pH 8.5-8.8 system. The discs were removed after electrophoresis, and the agar was overlaid with Bacillus subtilis spores in Mueller-Hinton agar. After incubation at 37 C, each individual antimicrobial a...

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Bibliographic Details
Published inAvian diseases Vol. 32; no. 2; p. 370
Main Author Freidlin, P.J
Format Journal Article
LanguageEnglish
Published United States 01.04.1988
Subjects
ANIMAL DISEASES
Anti-Bacterial Agents - analysis
ANTIMICROBIALS
ANTIMICROBIANO
ANTIMICROBIEN
AVES DE CORRAL
BACILLUS SUBTILIS
Bacillus subtilis - drug effects
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
Electrophoresis - methods
Electrophoresis - standards
ENFERMEDADES DE LOS ANIMALES
ESCHERICHIA
ESCHERICHIA COLI
IDENTIFICACION
IDENTIFICATION
MALADIE DES ANIMAUX
Microbial Sensitivity Tests
POULTRY
VOLAILLE
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Summary:Sensitivity discs were placed on 1% Noble agar for electrophoresis in a 20 mM phosphate-buffered, pH 8.5-8.8 system. The discs were removed after electrophoresis, and the agar was overlaid with Bacillus subtilis spores in Mueller-Hinton agar. After incubation at 37 C, each individual antimicrobial agent produced a distinctive identifying pattern of B. subtilis growth inhibition. The few antimicrobial agents with similar patterns could be easily differentiated by the use of a strain of Escherichia coli containing a multiple-resistance factor. Filter discs impregnated with commercial antibiotic preparations in buffer or serum yielded growth-inhibition patterns which usually resembled those obtained from corresponding sensitivity discs.
Bibliography:L74
8853211
ISSN:0005-2086
1938-4351
DOI:10.2307/1590829