A novel, efficient regenerating method of NADPH using a new formate dehydrogenase

A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) depende...

Full description

Saved in:
Bibliographic Details
Published inTetrahedron letters Vol. 37; no. 9; pp. 1377 - 1380
Main Authors Seelbach, Karsten, Riebel, Bettina, Hummel, Werner, Kula, Maria-Regina, Tishkov, Vladimir I., Egorov, Alexey M., Wandrey, Christian, Kragl, Udo
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 26.02.1996
Elsevier
Subjects
Chemistry
Chemistry, Organic
Physical Sciences
Science & Technology
TRANSFORMATION
CATALYZED ORGANIC-SYNTHESIS
ENZYME-MEMBRANE REACTOR
REDUCTION
ALCOHOL-DEHYDROGENASE
R(+)-PHENYLETHANOL
PURIFICATION
THERMOANAEROBIUM-BROCKII
METHYLOTROPHIC BACTERIUM
NAD(P)H COFACTOR REGENERATION
Online AccessGet full text

Cover

More Information
Summary:A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems. Graphic
ISSN:0040-4039
1873-3581
DOI:10.1016/0040-4039(96)00010-X