Interphase cytogenetics and comparative genomic hybridization of human epithelial cancers and precursor lesions

The accuracy of cytogenetic analyses of human solid cancers has improved enormously over the past decade by the introduction and refinement of DNA in situ hybridization (ISH) techniques. This methodology can be applied to cells in the interphase state, thereby making it an excellent tool for the del...

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Published inHistochemistry and cell biology Vol. 108; no. 4-5; pp. 419 - 430
Main Authors van Dekken, H, Rosenberg, C, Krijtenburg, P J, Alers, J C
Format Journal Article
LanguageEnglish
Published Germany Springer Nature B.V 01.10.1997
Subjects
Breast
Breast cancer
Breast carcinoma
Chromosome aberrations
Cytogenetics
Genetic analysis
Humans
Hybridization
In Situ Hybridization, Fluorescence - methods
Interphase
Invasiveness
Lung cancer
Lung carcinoma
Neoplasms, Glandular and Epithelial - genetics
Neoplasms, Glandular and Epithelial - pathology
Precancerous Conditions - genetics
Prostate cancer
Prostate carcinoma
Solid tumors
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Summary:The accuracy of cytogenetic analyses of human solid cancers has improved enormously over the past decade by the introduction and refinement of DNA in situ hybridization (ISH) techniques. This methodology can be applied to cells in the interphase state, thereby making it an excellent tool for the delineation of chromosomal aberrations in solid tumors. The use of non-isotopic ISH to intact and disaggregated cancer specimens will be discussed, as well as comparative genomic hybridization (CGH) with tumor-derived DNAs. In this review we will focus on hybridocytochemical interphase approaches for the detection of chromosomal changes in frequently occurring human epithelial malignancies, e.g., breast, lung, and prostate carcinomas. We will further discuss the use of ISH procedures for the genetic analysis of precursor conditions leading to invasive carcinomas. Knowledge concerning these precancerous conditions is increasing, and its importance in cancer prevention has been recognized. Interphase cytogenetics by ISH, as well as CGH, with DNAs derived from microdissected, precancerous, dysplastic tissue areas will increase our understanding of these lesions, both at the investigative and diagnostic levels.
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ISSN:0948-6143
1432-119X
DOI:10.1007/s004180050182