Determination of fluoxetine and norfluoxetine in human plasma by capillary gas chromatography with electron-capture detection
A capillary gas chromatographic method with 63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid—liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sens...
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Published in | Journal of chromatography Vol. 614; no. 1; pp. 175 - 179 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
21.04.1993
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | A capillary gas chromatographic method with
63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid—liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sensitivity of detection. A 30 m × 0.25 mm I.D. DB-17 capillary column resolves the compounds from endogenous matrix interferences. The limit of quantitation by this method is 5 ng/ml for each compound. Stability studies show that fluoxetine and norfluoxetine are stable in human plasma for up to 96 h at room temperature and up to one year at −20°C. |
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ISSN: | 0378-4347 |
DOI: | 10.1016/0378-4347(93)80239-Z |