Determination of fluoxetine and norfluoxetine in human plasma by capillary gas chromatography with electron-capture detection

A capillary gas chromatographic method with 63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid—liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sens...

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Published inJournal of chromatography Vol. 614; no. 1; pp. 175 - 179
Main Authors Lantz, R.J., Farid, K.Z., Koons, J., Tenbarge, J.B., Bopp, R.J.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 21.04.1993
Elsevier
Subjects
Analysis
Biological and medical sciences
Calibration
Chromatography, Gas - methods
Drug Stability
Electrons
Fluorocarbons
Fluoxetine - analogs & derivatives
Fluoxetine - blood
General pharmacology
Humans
Indicators and Reagents
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Toluene
Human
Validation
Electron capture detector
Biological fluid
Serotonin
Metabolite
Psychotropic
Uptake
Blood plasma
Assay
Gas chromatography
Antidepressant agent
Inhibitor
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Summary:A capillary gas chromatographic method with 63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid—liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sensitivity of detection. A 30 m × 0.25 mm I.D. DB-17 capillary column resolves the compounds from endogenous matrix interferences. The limit of quantitation by this method is 5 ng/ml for each compound. Stability studies show that fluoxetine and norfluoxetine are stable in human plasma for up to 96 h at room temperature and up to one year at −20°C.
ISSN:0378-4347
DOI:10.1016/0378-4347(93)80239-Z