Molecular Analysis of 11q13 Breakpoints in Multiple Myeloma

• Published in: Blood Vol. 93; no. 4; pp. 1330 - 1337
• Main Authors: Ronchetti, Domenica, Finelli, Palma, Richelda, Raffaella, Baldini, Luca, Rocchi, Mariano, Viggiano, Luigi, Cuneo, Antonio, Bogni, Silvia, Fabris, Sonia, Lombardi, Luigia, Maiolo, Anna Teresa, Neri, Antonino
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 15.02.1999; The Americain Society of Hematology
• Subjects: Base Sequence; Biological and medical sciences; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 14; Cloning, Molecular; Gene Rearrangement; Humans; Immunodeficiencies. Immunoglobulinopathies; Immunoglobulinopathies; Immunopathology; In Situ Hybridization, Fluorescence; Medical sciences; Molecular Sequence Data; Multigene Family; Multiple Myeloma - genetics; Translocation, Genetic; Chromosomal aberration; Human; Immunopathology; Breakpoint; Malignant hemopathy; Myeloma; Immunoglobulinopathy; Lymphoproliferative syndrome; Established cell line; Abnormal chromosome D14; Cytogenetics; Abnormal chromosome; Genetics; Molecular biology; Abnormal chromosome C11; Southern blotting; Chromosome translocation
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V93.4.1330

The t(11;14)(q13;q32) chromosomal translocation, which is the hallmark of mantle cell lymphoma (MCL), is found in approximately 30% of multiple myeloma (MM) tumors with a 14q32 translocation. Although the overexpression of cyclin D1 has been found to be correlated with MM cell lines carrying the t(11;14), rearrangements of theBCL-1/cyclin D1 regions frequently involved in MCL rarely occur in MM cell lines or primary tumors. To test whether specific 11q13 breakpoint clusters may occur in MM, we investigated a representative panel of primary tumors by means of Southern blot analysis using probes derived from MM-associated 11q13 breakpoints. To this end, we first cloned the breakpoints and respective germ-line regions from a primary tumor and the U266 cell line, as well as the germ-line region from the KMS-12 cell line. DNA from 50 primary tumors was tested using a large panel of probes, but a rearrangement was detected in only one case using the KMS-12 breakpoint probe. Our results confirm previous findings that the 11q13 breakpoints in MM are scattered throughout the 11q13 region encompassing the cyclinD1 gene, thus suggesting the absence of 11q13 breakpoint clusters in MM.