Genetic basis and mapping of the resistance to Rice yellow mottle virus. III. Analysis of QTL efficiency in introgressed progenies confirmed the hypothesis of complementary epistasis between two resistance QTLs

• Published in: Theoretical and applied genetics Vol. 103; no. 6-7; pp. 1084 - 1092
• Main Authors: AHMADI, N, ALBAR, L, PRESSOIR, G, PINEL, A, FARGETTE, D, GHESQUIERE, A
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.11.2001; Berlin Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; chromosome 7; Chromosomes; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Genomes; Hypotheses; Molecular and cellular biology; Oryza sativa; Pest resistance; Plant pathogens; Rice yellow mottle virus; RNA polymerase; Varietal selection. Specialized plant breeding, plant breeding aims; Viruses; Asia; Mexico; Africa; Virus; Genetic mapping; Resistance; Progeny; Rice yellow mottle virus; Sobemovirus; Efficiency; Analysis; Quantitative character; Epistasis; Hypothesis
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220100642

Our previous studies have hypothesised that a complementary epistasis between a QTL located on chromosome 12 and a QTL located on chromosome 7 was one of the major genetic factors controlling partial resistance to Rice yellow mottle virus (RYMV). We report research undertaken to verify this hypothesis and to introgress the resistant allele of these two QTLs from an upland resistant japonica variety, Azucena, into a lowland susceptible indica variety IR64. Three cycles of molecular marker-assisted back cross breeding were performed using RFLP and microsatellite markers. Resistance to RYMV was evaluated in F^sub 2^ and F^sub 3^ offspring of the BC^sub 1^ and BC^sub 2^ generations. Marker-assisted introgression (MAI) was very efficient: in the selected BC^sub 3^ progeny the proportion of the recipient genome was close to 95% for the ten non-carrier chromosomes, and the length of the donor chromosome segment surrounding the two QTLs was less than 20 cM. The relevancy of the complementary epistasis genetic model proposed previously was confirmed experimentally: in BC^sub 1^ and BC^sub 2^ generations only F3 lines having the allele of the resistant parent on QTL^sub 12^ and QTL^sub 7^ show partial resistance to RYMV. Comparison of our experimental process of MAI with the recommendations of analytic and simulation studies pointed out the methodological flexibility of MAI. Our results also confirmed the widely admitted, but rarely verified, assumption that QTL-alleles detected in segregating populations could be treated as units of Mendelian inheritance and that the incorporation of these alleles into elite lines would result in an enhanced performance. The next step will be the design of tools for the routine use of molecular markers in breeding for partial resistance to RYMV and the development of material for the analysis of resistance mechanisms and the structure of a virus resistance gene in rice.[PUBLICATION ABSTRACT]