Immunoaffinity chromatography combined with gas chromatography—negative ion chemical ionisation mass spectrometry for the confirmation of flumethasone abuse in the equine

Immunoaffinity chromatography using a synthesised immunosorbent was used to extract tritiated dexamethasone (with dexamethasone carrier) from equine urine at a recovery of 81.7 ± 8.4% (mean ± S.D.). A method utilising this procedure coupled to cool on-column injection gas chromatography—negative ion...

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Bibliographic Details
Published inJournal of chromatography Vol. 614; no. 1; pp. 77 - 86
Main Authors Stanley, S.M.R., Wilhelmi, B.S., Rodgers, J.P., Bertschinger, H.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 21.04.1993
Elsevier
Subjects
Analysis
Animals
Antibodies
Biological and medical sciences
Chromatography, Affinity - methods
Cross Reactions
Dexamethasone
Doping in Sports
Flumethasone - urine
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Horses
Hydrocortisone
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Urine
Drug abuse
Corticosteroid
Biological fluid
Perfecting
Immunoaffinity chromatography
Steroid hormone
Doping
Characterization
Assay
Gas chromatography
Vertebrata
Mammalia
Animal
Horse
Adrenal hormone
Perissodactyla
Mass spectrometry
Ungulata
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Summary:Immunoaffinity chromatography using a synthesised immunosorbent was used to extract tritiated dexamethasone (with dexamethasone carrier) from equine urine at a recovery of 81.7 ± 8.4% (mean ± S.D.). A method utilising this procedure coupled to cool on-column injection gas chromatography—negative ion chemical ionisation mass spectrometry is also described for the confirmation of low levels of flumethasone in equine urine samples.
ISSN:0378-4347
DOI:10.1016/0378-4347(93)80225-S