Progress in single-molecule spectroscopy in cells

Time-dependent single-molecule spectroscopy allows the direct observation of molecular processes in real time, capturing transient and stochastic events that cannot be detected using conventional ensemble-averaged methods. It offers a promising approach to uncovering the physical and chemical transf...

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Bibliographic Details
Published inCurrent opinion in chemical biology Vol. 14; no. 1; pp. 3 - 9
Main Author Yang, Haw
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.02.2010
Subjects
Cells - cytology
Cells - metabolism
Macromolecular Substances - chemistry
Molecular Probe Techniques
Nanostructures - chemistry
Quantum Dots
Spectrometry, Fluorescence
Time Factors
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Summary:Time-dependent single-molecule spectroscopy allows the direct observation of molecular processes in real time, capturing transient and stochastic events that cannot be detected using conventional ensemble-averaged methods. It offers a promising approach to uncovering the physical and chemical transformations underlying cellular responses. Recent advances in the probe development, specific labeling strategies, and spectroscopy instrumentation suggest the feasibility of monitoring a single molecular machine's actions inside a living cell.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ObjectType-Review-2
ISSN:1367-5931
1879-0402
DOI:10.1016/j.cbpa.2009.10.015