Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) with and without ethidium bromide. Constants of Mt2...

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Published inInternational journal of biological macromolecules Vol. 55; pp. 137 - 141
Main Authors Sorokin, V.A., Valeev, V.A., Usenko, E.L., Rakovsky, Ya.P., Andrushchenko, V.V.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.04.2013
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Summary:Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) with and without ethidium bromide. Constants of Mt2+ binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency of Zn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10°С upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strong cooperative decrease (up to 30°С) of the temperature of В→m transition induced by heating in the presence of Zn2+ was found and its nature was explained.
Bibliography:http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001
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ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2013.01.001