The androgenic regulation of abundant mRNA in rat ventral prostate
The most abundant poly(A)‐containing RNA accounting for approximately half the total is regulated by testosterone in rat ventral prostate. A complementary DNA probe to these sequences was isolated by hybridizing the total cellular poly(A)‐containing RNA with its complementary DNA to an rot1/2 value...
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Published in | European journal of biochemistry Vol. 85; no. 2; pp. 399 - 406 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
17.04.1978
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Subjects | |
Online Access | Get full text |
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Summary: | The most abundant poly(A)‐containing RNA accounting for approximately half the total is regulated by testosterone in rat ventral prostate. A complementary DNA probe to these sequences was isolated by hybridizing the total cellular poly(A)‐containing RNA with its complementary DNA to an rot1/2 value of 2 × 10−2 mol l−1 s and separating the abundant cDNA, from the non‐abundant cDNA by hydroxyapatite chromatography. The abundant cDNA, complementary to about three different poly(A)‐containing RNA sequences comprising 45% of the total poly(A)‐containing RNA, has been used to investigate the effect of androgens on their metabolism.
Following castration there is a progressive decrease in the abundant sequences from 45% of the total in normal animals to less than 0.03% after 14 days; testosterone administration in vivo results in their regeneration. The sequences were not detected in seminal vesicle, liver, heart or spleen but represented about 0.01% of the poly(A)‐containing RNA of kidney.
Translation of the poly(A)‐containing RNA in vitro in a cell‐free system derived from wheat germ resulted in the synthesis of four major proteins which could be separated by polyacrylamide gel electrophoresis. Their synthesis, as measured by [35S]methionine incorporation, accounted for 40 – 50% of the total incorporation and declined after castration but was restored by testosterone treatment. We conclude that a class of àbundant poly(A)‐containing RNA which codes for four major proteins is regulated by testosterone in rat ventral prostate. |
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Bibliography: | 784X822 L50 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-2956 1432-1033 |
DOI: | 10.1111/j.1432-1033.1978.tb12252.x |