Antioxidant effect of flavonoids on DCF production in HL-60 cells

• Published in: Phytotherapy research Vol. 17; no. 8; pp. 963 - 966
• Main Authors: Takamatsu, Satoshi, Galal, Ahmed M., Ross, Samir A., Ferreira, Daneel, ElSohly, Mahmoud A., Ibrahim, Abdel-Rahim S., El-Feraly, Farouk S.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2003
• Subjects: antioxidants; Antioxidants - administration & dosage; Antioxidants - pharmacology; Antioxidants - therapeutic use; DCFH oxidation; Dose-Response Relationship, Drug; Flavonoids - administration & dosage; Flavonoids - pharmacology; Flavonoids - therapeutic use; Flow Cytometry; Fluoresceins - chemistry; HL-60 cell; HL-60 Cells - drug effects; Humans; Oxidation-Reduction; Phytotherapy; Plants, Medicinal; Respiratory Burst - drug effects; respiratory burst activity; flavonoids
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.1289

A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL‐60 by assessing oxidation of 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1–18) were evaluated. Copyright © 2003 John Wiley & Sons, Ltd.