Thrombin-hirudin complex stability: a comparison with the thrombin-antithrombin III complex

In the present in vitro study the stabilities of the thrombin-hirudin and the thrombin-antithrombin III complexes were investigated. After incubation of the complexes with free inhibitors the thrombin-antithrombin III levels were determined by ELISA. The thrombin-hirudin complex proved to be stable...

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Bibliographic Details
Published inBlood coagulation & fibrinolysis Vol. 2; no. 5; p. 643
Main Authors Römisch, J, Pâques, E P
Format Journal Article
LanguageEnglish
Published England 01.10.1991
Subjects
Antithrombin III - chemistry
Drug Stability
Heparin - chemistry
Hirudins - chemistry
Humans
Peptide Hydrolases - chemistry
Thrombin - chemistry
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Summary:In the present in vitro study the stabilities of the thrombin-hirudin and the thrombin-antithrombin III complexes were investigated. After incubation of the complexes with free inhibitors the thrombin-antithrombin III levels were determined by ELISA. The thrombin-hirudin complex proved to be stable in the presence of antithrombin III or heparin. However, in the presence of heparin and plasma equivalent concentrations of antithrombin III, the thrombin-hirudin complex dissociated and hirudin was displaced. In contrast, both thrombin-antithrombin III and thrombin-antithrombin III/heparin complexes are very stable even in the presence of a large excess of hirudin.
ISSN:0957-5235
DOI:10.1097/00001721-199110000-00009