Evaluation of column characteristics in RPLC using linear solvation energy relationships (LSERs)

• Published in: Journal of separation science Vol. 26; no. 3-4; pp. 201 - 214
• Main Author: Szepesy, László
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.03.2003; WILEY‐VCH Verlag; Wiley
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Linear solvation energy relationships; Narrow pore and wide pore packings; Other chromatographic methods; Retention and selectivity factors; Various ligands; Phase composition; Property composition relationship; Chromatographic retention; Retention and selectivity factors; Benzene derivatives; HPLC chromatography; Various ligands; Selectivity; Regression analysis; Review; Mobile phase; Linear solvation energy relationships; Regression coefficient; Reversed phase chromatography; Solvation; Correlation analysis; Narrow pore and wide pore packings; Bonded stationary phase
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200390027

An overview is given of our studies on the use of linear solvation energy relationships (LSERs) for evaluation and comparison of RP‐HPLC columns. From among the columns investigated, 19 columns of different types, viz. 12 narrow‐pore, 7 wide‐pore silicas containing various ligands (C18, C8, C4, CN) and obtained from 7 manufacturers, were compared and evaluated. Retention factors of 32 solutes of widely differing chemical properties were measured and evaluated to determine the effect of stationary phase properties on the retention of various types of solutes. When the same mobile phase is used with the different columns, the regression coefficients determined by multivariate regression characterize the various stationary phases. The columns investigated were evaluated on the basis of the relative importance of the various types of interactions reflecting the various surface properties of the stationary phases. Since the selectivity of separation is more important than is absolute selectivity, we have investigated and compared the various selectivity terms, hydrophobic, polar, and specific selectivity, displayed by the various columns, for the separation of different types of compounds. As the selectivity of separation depends also on the type and composition of the mobile phase, the effect of mobile phase on the various selectivity terms was evaluated.