HPLC of humic substances fractionated by Flow FFF

This communication reports a study of the effect of ionic strength and electrolyte composition on fractions, separately collected by flow FFF, of a mixture of humic substances. Reverse phase HPLC analysis of three early eluting fractions suggests that the components released by the column behave as...

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Bibliographic Details
Published inJournal of separation science Vol. 26; no. 5; pp. 429 - 432
Main Authors Benincasa, Maria-Anna, Cartoni, Giampaolo, Imperia, Nadia
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.04.2003
WILEY‐VCH Verlag
Wiley
Subjects
Agronomy. Soil science and plant productions
Biological and medical sciences
Chemical, physicochemical, biochemical and biological properties
Flow field-flow fractionation
Fundamental and applied biological sciences. Psychology
HPLC
Humic substances
Organic matter
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Soil science
Field flow fractionation
Organic matter
Chemical analysis
Phase composition
Multicomponent mixture
HPLC chromatography
Mobile phase
Humic substances
Separation method
Reversed phase chromatography
Ultraviolet detector
Analysis method
pH
Flow field-flow fractionation
Humic acid
HPLC
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Summary:This communication reports a study of the effect of ionic strength and electrolyte composition on fractions, separately collected by flow FFF, of a mixture of humic substances. Reverse phase HPLC analysis of three early eluting fractions suggests that the components released by the column behave as organic acids. The baseline‐resolved peaks of the first two fractions, subject to higher retention in solutions of lower pH and/or higher polarity, substantiate this suggestion. The fraction with larger components, as measured by flow FFF, also appears to contain acidic species. Their retention level, however, may not be accurately modulated by varying the mobile phase properties as these species are either totally retained in acidic phases or released before the void peak at pH ≥ 4.2. Besides showing the effective separation achieved in the flow FFF channel, this study reveals the pronounced difference in the physicochemical properties of some components of a humic mixture even with very close particle size.
Bibliography:istex:84EE66075CF148B464DBDE2C6138250BD8E7AB53
ArticleID:JSSC200390056
ark:/67375/WNG-MX0D1HNM-X
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200390056