Capillary electrochromatographic separation of ionizable compounds with a molecular imprinted monolithic cationic exchange column

• Published in: Journal of separation science Vol. 26; no. 6-7; pp. 555 - 561
• Main Authors: Yan, Weiying, Gao, Ruyu, Zhang, Zhichao, Wang, Qinsun, Jiang, Chun Vernon, Yan, Chao
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.05.2003; WILEY‐VCH Verlag; Wiley
• Subjects: 2-Aminopyridine; 4-Aminopyridine; Analytical chemistry; Capillary electrochromatography; Cationic exchange column; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Ion exchange chromatography; Ionizable compounds; Molecular imprinted polymer; Monolithic column; Other chromatographic methods; Structural isomers; Separation; 2-Aminopyridine; Phase composition; Nitrogen heterocycle; Molecular imprinted polymer; Monolithic column; Capillary electrochromatography; Experimental study; Mobile phase; Ion exchange chromatography; Crosslinked copolymer; Ultraviolet detector; Position isomer; 4-Aminopyridine; Electrophoretic mobility; Electrochromatography; Voltage; pH; Cationic exchange column; Methacrylate copolymer; Structural isomers; Molecular imprinting; lonizable compounds; Cation exchanger
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200390076

A polymer‐based monolithic capillary column imprinted with 4‐aminopyridine (4‐AP) was prepared by a thermally‐initiated polymerization process; and its performance as a capillary electrochromatographic medium was evaluated in separating 4‐AP and 2‐AP isomers. The effects of experimental parameters, such as pH value and ionic strength of the buffer, the acetonitrile content in the mobile phase, and the applied voltage, on the resolution of these isomers had been carefully investigated. It was found that in the retention process there were interplays of multiple mechanisms of ion‐exchange, molecular imprinting, and electrophoresis. These mechanisms allowed more sophisticated control of experimental parameters in the separation of ionizable compounds.