Age-related changes of the endogenous cardiolipin and plasmalogens of guinea pig kidney and their in vitro hydrolysis by endogenous phospholipases: a thin layer chromatographic analysis in conjunction with densitometric measurement

• Published in: Cell biochemistry and function Vol. 21; no. 4; pp. 337 - 344
• Main Authors: Helmy, Fatma M., Hack, Marvin H., Juracka, Amal
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.12.2003
• Subjects: A2 (endogenous); Aging - physiology; Animals; cardiolipin; Cardiolipins - chemistry; Cardiolipins - metabolism; Chromatography, Thin Layer; Densitometry; guinea pig kidney (pre-post natal); Guinea Pigs; Hydrolysis; Kidney - metabolism; lyso alkenyl phosphatidyl choline; lyso alkenyl phosphatidyl ethanolamine; monolysocardiolipin; Phosphatidylglycerols - metabolism; phospholipase A1; phospholipase A1, A2 (endogenous); Phospholipases - metabolism; plasmalogens
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1035

The phosphoglycerides profile of guinea pig kidney, fetal, young adult, and aged, and their in vitro response to the endogenous lipolytic enzymes, mainly in the phospholipase group were determined by TLC technology in conjunction with densitometric measurement. Changes in phosphoglycerides profile subsequent to in vitro incubation of these tissues at pH 7.4, and 38°C for 45 min and prior to phospholipid extraction has provided evidence relating to their respective lipolytic enzymes capabilities and age. These changes are mainly related to endogenous cardiolipin (CL), alkenyl phospholipids (phosphatidyl ethanolamine and phosphatidyl choline) and their endogenous deacylation to their respective lyso derivatives monolysocardiolipin (MLCL), lyso alkenyl phosphatidyl ethanolamine (LPE), and lyso alkenyl phosphatidyl choline (LPC) by endogenous phospholipases. The hydrolysis of the plasmalogen confirms the action of endogenous PLA2 on sn‐2 fatty acids of these compounds. Copyright © 2003 John Wiley & Sons, Ltd.