Molecular interactions between sugar beet and Polymyxa betae during its life cycle

• Published in: Annals of applied biology Vol. 164; no. 2; pp. 244 - 256
• Main Authors: Desoignies, N, Carbonell, J, Moreau, J.‐S, Conesa, A, Dopazo, J, Legrève, A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2014; Blackwell; Wiley Subscription Services, Inc
• Subjects: Beet necrotic yellow vein virus; beets; Biological and medical sciences; blood coagulation factors; expressed sequence tags; Fundamental and applied biological sciences. Psychology; genes; Genomics; lectins; Parasites; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Plasmodiophorid; Polymyxa betae; Proteins; rhizomania; roots; stress tolerance; sugar beet; Sugar industry; suppression subtractive hybridization; viruses; Plant production; Molecular hybridization; Protozoa; Plant pathogen; RNA virus; rhizomania; Molecular interaction; Polymyxa betae; Cycle; Virus; Suppression subtractive hybridization; Applied biology; Plasmodiophorid; Sugar beet; Benyvirus; Mycetozoa; Beet necrotic yellow vein virus
• ISSN: 0003-4746; 1744-7348
• DOI: 10.1111/aab.12095

Polymyxa betae is a biotrophic obligate sugar beet parasite that belongs to plasmodiophorids. The infection of sugar beet roots by this parasite is asymptomatic, except when it transmits Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania. To date, there has been little work on P. betae–sugar beet molecular interactions, mainly because of the obligate nature of the parasite and also because research on rhizomania has tended to focus on the virus. In this study, we investigated these interactions through differential transcript analysis, using suppressive subtractive hybridization. The analysis included 76 P. betae and 120 sugar beet expressed sequence tags (ESTs). The expression of selected ESTs from both organisms was monitored during the protist life cycle, revealing a potential role of two P. betae proteins, profilin and a Von Willebrand factor domain‐containing protein, in the early phase of infection. This study also revealed an over‐expression of some sugar beet genes involved in defence, such as those encoding PR proteins, stress resistance proteins or lectins, especially during the plasmodial stage of the P. betae life cycle. In addition to providing new information on the molecular aspects of P. betae–sugar beet interactions, this study also enabled previously unknown ESTs of P. betae to be sequenced, thus enhancing our knowledge of the genome of this protist.