Inhibitory effect of tenomodulin versus ranibizumab on in vitro angiogenesis

AIM： To evaluate anti-angiogenic effect of tenomodulin （TNMD） and ranibizumab on cell proliferation and capillary- like morphogenesis of vascular endothelial cells under the stimulation of vascular endothelial growth factor （VEGF） in vitro. METHODS： The effects of TNMD and ranibizumab on VEGF-induce...

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Published inInternational journal of ophthalmology Vol. 10; no. 8; pp. 1212 - 1216
Main Authors Wang, Wei, Liu, Guang-Xu, Li, Yue-Hua, Li, Xue-Dong, He, Yan
Format Journal Article
LanguageEnglish
Published China International Journal of Ophthalmology Press 18.08.2017
Press of International Journal of Ophthalmology (IJO PRESS)
Subjects
1216
angiogenesis
Basic Research
inhibitory effect
proliferation
ranibizumab
tenomodulin
tenomodulin
inhibitory effect
proliferation
angiogenesis
ranibizumab
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Summary:AIM： To evaluate anti-angiogenic effect of tenomodulin （TNMD） and ranibizumab on cell proliferation and capillary- like morphogenesis of vascular endothelial cells under the stimulation of vascular endothelial growth factor （VEGF） in vitro. METHODS： The effects of TNMD and ranibizumab on VEGF-induced proliferation of human umbilical vein endothelial cells （HUVECs） were evaluated by MTT assay, and the effects of TNMD and ranibizumab on capillary- like structures formed by HUVECs under the stimulation of VEGF were examined in culture. Capillary-like morphogenesis of HUVECs was quantitatively evaluated, and total lengths of tube-like structures per field were measured in a masked way. RESULTS： HUVECs with both ranibizumab and TNMD protein showed MTT reduction in VEGF-stimulated cell proliferation as expected, while MTT absorbance in the HUVECs with TNMD was significantly declined than that with ranibizumab （P〈O.01）. The capillary-like structures formed by HUVECs were markedly impaired by the presence of both TNMD and ranibizumab in the culture medium. The total length of the capillary-like structures per field was significantly shorter in the medium with TNMD than that of ranibizumab （P〈0.01）. The inhibitory effect of TNMD on tube formation in vitro angiogenesis was significantly stronger than that of ranibizumab. CONCLUSION： TNMD may have stronger inhibitory effect than ranibizumab on in vitro angiogenesis.
Bibliography:tenomodulin; ranibizumab; inhibitory effect; proliferation; angiogenesis
AIM： To evaluate anti-angiogenic effect of tenomodulin （TNMD） and ranibizumab on cell proliferation and capillary- like morphogenesis of vascular endothelial cells under the stimulation of vascular endothelial growth factor （VEGF） in vitro. METHODS： The effects of TNMD and ranibizumab on VEGF-induced proliferation of human umbilical vein endothelial cells （HUVECs） were evaluated by MTT assay, and the effects of TNMD and ranibizumab on capillary- like structures formed by HUVECs under the stimulation of VEGF were examined in culture. Capillary-like morphogenesis of HUVECs was quantitatively evaluated, and total lengths of tube-like structures per field were measured in a masked way. RESULTS： HUVECs with both ranibizumab and TNMD protein showed MTT reduction in VEGF-stimulated cell proliferation as expected, while MTT absorbance in the HUVECs with TNMD was significantly declined than that with ranibizumab （P〈O.01）. The capillary-like structures formed by HUVECs were markedly impaired by the presence of both TNMD and ranibizumab in the culture medium. The total length of the capillary-like structures per field was significantly shorter in the medium with TNMD than that of ranibizumab （P〈0.01）. The inhibitory effect of TNMD on tube formation in vitro angiogenesis was significantly stronger than that of ranibizumab. CONCLUSION： TNMD may have stronger inhibitory effect than ranibizumab on in vitro angiogenesis.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2222-3959
2227-4898
DOI:10.18240/ijo.2017.08.04