Establishment and characterization of a cell line (IGSK-3) secreting human chorionic gonadotropin, adrenocorticotropic hormone and parathyroid hormone-related protein derived from primary poorly differentiated adenocarcinoma of the stomach

• Published in: Human cell : official journal of Human Cell Research Society Vol. 21; no. 3; pp. 88 - 94
• Main Authors: Yamashita, Shigeo, Takahashi, Naoto, Hashimoto, Hisashi, Tachibana, Toshiaki, Nakahara, Taka, Ohyama, Akihiro, Yanaga, Katsuhiko
• Format: Journal Article
• Language: English
• Published: Tokyo Springer-Verlag 01.08.2008; Blackwell Publishing Asia
• Subjects: ACTH; Adenocarcinoma - genetics; Adenocarcinoma - pathology; Adenocarcinoma - secretion; Adenocarcinoma - ultrastructure; Adrenocorticotropic Hormone - secretion; Animals; Biomedical and Life Sciences; Cell Biology; Cell Line; Cell Line, Tumor; Chorionic Gonadotropin - secretion; epidermal growth factor receptor (EGFR); Female; Gynecology; hCG‐β; Humans; Karyotyping; Life Sciences; Mice; Mice, Nude; Microscopy, Phase-Contrast; Middle Aged; Neoplasm Transplantation; Oncology; Parathyroid Hormone-Related Protein - secretion; poorly differentiated adenocarcinoma; PTHrp; Reproductive Medicine; Stem Cells; stomach; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Stomach Neoplasms - secretion; Stomach Neoplasms - ultrastructure; Surgery; Transplantation, Heterologous; poorly differentiated adenocarcinoma; epidermal growth factor receptor (EGFR); ACTH; hCG-β; stomach; PTHrp; cell line
• ISSN: 0914-7470; 1749-0774
• DOI: 10.1111/j.1749-0774.2008.00054.x

We recently established human chorionic gonadotropin-, adrenocorticotropic hormone- and parathyroid hormone-related protein-secreting cell line derived from primary poorly differentiated adenocarcinoma of the stomach. The cell line was designated as IGSK-3. Inverted-phase contrast microscopy revealed that the IGSK-3 cells consist of two morphological subtypes. One type has visible nucleoli and clear nuclei, but nucleoli and nuclear membrane of the othertype are invisible. The population-doubling time was about 43 h. An analysis of conditioned medium by IGSK-3 cells cultured for 4 days revealed the IGSK-3 cells secrete human chorionic gonadotropin-β (0.5 ng/mL), adrenocorticotropic hormone (5.5 pg/mL), parathyroid hormone-related protein (3.4 pmol/mL) and epidermal growth factor (14.2 pg/mL). Histopathological diagnosis of the graft of IGSK-3 cells revealed that IGSK-3 cells built a poorly differentiated adenocarcinoma which resembled the original tumor. In addition, the IGSK-3 cell line was immunocytochemically positive for human chorionic gonadotropin-β and epidermal growth factor receptor, and negative for vascular endothelial growth factor.