CELL PROLIFERATION AND DIFFERENTIATION OF CANCEROUS AND NON-CANCEROUS THYROID TISSUES OF RATS

• Published in: ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 24; no. 2; pp. 173 - 179
• Main Authors: IMAMURA, YOSHIAKI, SUGIHARA, HIROYUKI, NORIKI, SAKON, MIYOSHI, NORIO, NAKANISHI, KAZUO, FUKUDA, MASARU
• Format: Journal Article
• Language: English
• Published: Kyoto JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 1991; Japan Society of Histochemistry and Cytochemistry; Japan Science and Technology Agency
• Subjects: Biological and medical sciences; Cell structures and functions; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Tissue; Vertebrata; Mammalia; Rat; Rodentia; Tumor; Thyroid gland; Cell differentiation
• ISSN: 0044-5991; 1347-5800
• DOI: 10.1267/ahc.24.173

The cell proliferative activity and capability of cytodifferentiation of cancerous and non-cancerous thyroid tissues were studied using 3H-thymidine (3H-TdR) autoradiography and immunohistochemistry for thyroglobulin. Cancerous lesions were induced by diisopropanolnitrosamine (DIPN) followed by methylthiouracil (MTU). Regenerative thyroid tissues were obtained through wounding. All the rats treated successively with DIPN and MTU were found to have carcinomas that invaded the surrounding tissue or blood vessels. By cumulative labeling with 3H-TdR, the maximal cell cycle times (Tc) of invasive and non-invasive parts of carcinomas were estimated to be 120hr and 180hr, respectively, whereas the Tc of regenerative and hyperplastic thyroid tissues were estimated as 113hr and 519hr, respectively. However, the duration of S phase (Ts) was greater in cancers (15-35hr) than in non-cancerous tissues (3-4hr), and the cancerous tissues showed lower expression of thyroglobulin than the non-cancerous thyroid tissues. These may reflect some injury in the DNA of cancer cells.