Sodium crocetinate does not alter gut hypercapnic responses or renal energy stores during transient sub-diaphragmatic ischaemia

• Published in: Intensive care medicine Vol. 29; no. 4; pp. 652 - 654
• Main Authors: MORGAN, Thomas J, VENKATESH, Balasubramanian, CRERAR-GILBERT, Agnieszka, WILLGOSS, Desley, ENDRE, Zoltan H
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.04.2003; Berlin Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Carbon Dioxide - metabolism; Carotenoids - pharmacology; Disease Models, Animal; Hypercapnia - drug therapy; Hypercapnia - metabolism; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Ischemia - drug therapy; Ischemia - metabolism; Kidney - metabolism; Male; Medical sciences; Pneumology; Prospective Studies; Rats; Rats, Sprague-Dawley; Respiratory system : syndromes and miscellaneous diseases; Ischaemia; Animal model; Rat; Respiratory disease; Rodentia; Carbon dioxide; Gut; Cardiovascular disease; Experimental study; Kidney; Hypercapnia; Vertebrata; Mammalia; Ischemia; Sodium; PCO2 sensor; Animal; Adenine nucleotides; Crocetin; Quantitative analysis
• ISSN: 0342-4642; 1432-1238
• DOI: 10.1007/s00134-003-1641-2

To evaluate the protection afforded by trans-sodium crocetinate against dysoxia in an animal model of recurrent sub-diaphragmatic ischaemia. Prospective experimental animal study. University research laboratory Adult male Sprague-Dawley rats. Twelve adult male Sprague-Dawley rats (340-510 g) were anaesthetised with sodium pentobarbitone 60 mg/kg i.p. and ventilated with oxygen and isoflurane via tracheostomy. Six 2-min episodes of sub-diaphragmatic hypotension (mean pressure 30 mmHg) were induced using a sling around the proximal aorta. Before the third and sixth episodes, saline 1.5 ml/kg was injected into the aortic cannula. In six rats, this saline contained trans-sodium crocetinate 50 microg/ml. Ileal luminal PCO(2) and distal aortic pressure were monitored continuously. Following ischaemic episodes trans-sodium crocetinate had no discernible effect on either degree of PCO(2) elevation or the time to peak PCO(2). No effects on renal energy charge or nucleotide concentrations were detected. UV-visible spectroscopy of the crocetinate preparation showed that some cis isomer was present. The findings, although limited to one drug dosage in one animal model, bring into question whether trans-sodium crocetinate affects plasma oxygen diffusivity in vivo. Alternative explanations for the negative findings include a TSC-induced exacerbation of arterio-venous oxygen shunting, the brevity of the dysoxic episodes, and the presence of cis isomer.