DNA sequencing with blackbody infrared radiative dissociation of electrosprayed ions

As shown by Williams for positive multiply-charged ions, negative electrosprayed ions trapped in a Fourier-transform ion cyclotron resonance mass spectrometer can be dissociated efficiently with BIRD. For a 50-mer DNA, an ion cell wall temperature of 90°C gives no dissociation and 120°C gives only u...

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Bibliographic Details
Published inInternational journal of mass spectrometry and ion processes Vol. 167; pp. 705 - 712
Main Authors Aaserud, David J., Guan, Ziqiang, Little, Daniel P., McLafferty, Fred W.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.11.1997
Subjects
Blackbody infrared radiative dissociation
DNA
Electrospray
Fourier-transform ion cyclotron resonance
Blackbody infrared radiative dissociation
DNA
Electrospray
Fourier-transform ion cyclotron resonance
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Summary:As shown by Williams for positive multiply-charged ions, negative electrosprayed ions trapped in a Fourier-transform ion cyclotron resonance mass spectrometer can be dissociated efficiently with BIRD. For a 50-mer DNA, an ion cell wall temperature of 90°C gives no dissociation and 120°C gives only uninformative base loss. However, 150°C gives extensive dissociation producing ionic products qualitatively similar to those from laser IR, collisionally activated, and nozzle-skimmer dissociation. Under the conditions used, BIRD produces more fragment ions that have lost an additional base (AH). Structurally informative BIRD spectra are also observed for a 100-mer DNA and a double-strand 64-mer DNA. For both samples, BIRD successfully ‘boiled off’ non-covalent adducts that produced an uninterpretable spectrum, while laser IR was only successful in this for the ds 64-mer.
ISSN:0168-1176
1873-2801
DOI:10.1016/S0168-1176(97)00130-4