Differential Protection of Chickens against Highly Pathogenic H5 Avian Influenza Virus Using Polybasic Amino Acids with H5 Cleavage Peptide

• Published in: Frontiers in bioscience (Landmark. Print) Vol. 29; no. 1; p. 11
• Main Author: Seo, Sang Heui
• Format: Journal Article
• Language: English
• Published: Singapore IMR Press 16.01.2024
• Subjects: Amino Acids - metabolism; Animals; Chickens - metabolism; Dogs; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - metabolism; highly pathogenic avian influenza virus; Humans; Influenza A virus; Influenza in Birds - prevention & control; peptide; Peptides; vaccine; highly pathogenic avian influenza virus; peptide; vaccine
• ISSN: 2768-6701; 2768-6698
• DOI: 10.31083/j.fbl2901011

Highly pathogenic H5Nx viruses cause avian influenza, a zoonotic disease that can infect humans. The vaccine can facilitate the prevention of human infections from infected poultry. Our previous study showed that an H5 cleavage-site peptide vaccine containing the polybasic amino acid RRRK could protect chickens from lethal infections of the highly pathogenic H5N6 avian influenza virus. Chickens immunized with the various polybasic amino combinations (RRRK, RRR, RR, R, RK, and K) of H5 cleavage-site peptides were challenged with highly pathogenic H5N6 avian influenza viruses. The challenged chickens were monitored for survival rate, and viral titers in swabs and tissue samples were measured in Madin-Darby canine kidney (MDCK) cells using the median tissue culture infectious dose 50 (log10 TCID50/mL). Most H5 cleavage-site vaccines containing various combinations of polybasic amino acids protected chickens from lethal infection. Chickens immunized with the RK-containing peptide combination of the H5 cleavage site were not protected. The polybasic amino acids (RRRK) of H5 cleavage cleavage-site peptide vaccines are important for protecting chickens against HP H5N6 avian influenza virus. The H5 cleavage cleavage-site peptide containing RK did not protect chickens against the virus.