Re-examination of the taxonomic position of Bacillus silvestris Rheims et al. 1999 and proposal to transfer it to Solibacillus gen. nov. as Solibacillus silvestris comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 5; pp. 1054 - 1058
• Main Authors: Krishnamurthi, Srinivasan, Chakrabarti, Tapan, Stackebrandt, Erko
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.05.2009; Society for General Microbiology
• Subjects: Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cell Wall - chemistry; DNA, Bacterial - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Lipids - analysis; Microbiology; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Species Specificity; Spores, Bacterial; Systematics; Taxonomic review; New genus; Bacillales; Taxonomy; Bacillus; Bacteria; New species; Bacillaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65742-0

1 Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7b, D-38124 Braunschweig, Germany Correspondence Tapan Chakrabarti tapan{at}imtech.res.in Following the transfer of three of the six species enclosed in the original definition of rRNA group 2 of Bacillus to the genus Sporosarcina and two to Lysinibacillus , other species of this group, some of which were added later, still await taxonomic revision. In a recent publication, a set of ‘core’ characteristics was proposed for species to be included in the genus Bacillus (Kämpfer et al. , 2006). Except for Bacillus silvestris , however, several or none of these properties are available for members of rRNA group 2. According to our analysis of data including the ‘core’ characteristics, Bacillus silvestris should not be a member of the genus Bacillus . We therefore propose the establishment of a new genus, Solibacillus gen. nov., and transfer Bacillus silvestris to this genus as Solibacillus silvestris comb. nov., with the type strain HR3-23 T (=DSM 12223 T =ATCC BAA-269 T =CIP 106059 T ). Abbreviations: AL, aminolipid; APL, aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; PGL, phosphoglycolipid; PL, phospholipid Supplementary tables showing 16S rRNA gene sequence similarity between Bacillus silvestris and other closely related members of Bacillus rRNA group 2, and comparative fatty acid profiles of B. silvestris , Caryophanon latum and Bacillus subtilis are available with the online version of this paper.