Crystalline Human Urokinase: Some Properties

Human urokinase, an enzyme which activates plasminogen, has been crystallized. Repeated recrystallization yielded preparations of maximum specific activity; such preparations revealed only one component when subjected to polyacrylamide gel disc electrophoresis. Studies with the ultracentrifuge demon...

Full description

Saved in:
Bibliographic Details
Published inScience (American Association for the Advancement of Science) Vol. 147; no. 3660; pp. 880 - 882
Main Authors Lesuk, Alex, Terminiello, Louis, Traver, Janet H.
Format Journal Article
LanguageEnglish
Published United States American Association for the Advancement of Science 19.02.1965
Subjects
Cilia
Crystallization
Crystallography
Dendrites
Deoxyribonuclease I
Electrophoresis
Enzymes
Gels
Humans
Molecular Weight
Neurons
Neurosecretory cells
Old Medline
Streptodornase and Streptokinase
Streptokinase
Symbolism
Ultracentrifugation
Urokinase-Type Plasminogen Activator
CRYSTALLIZATION
ELECTROPHORESIS
STREPTODORNASE AND STREPTOKINASE
EXPERIMENTAL LAB STUDY
CRYSTALLOGRAPHY
MOLECULAR WEIGHT
ULTRACENTRIFUGATION
Online AccessGet full text

Cover

More Information
Summary:Human urokinase, an enzyme which activates plasminogen, has been crystallized. Repeated recrystallization yielded preparations of maximum specific activity; such preparations revealed only one component when subjected to polyacrylamide gel disc electrophoresis. Studies with the ultracentrifuge demonstrated that urokinase undergoes a concentration-dependent reversible association at pH 6.8. When the partial specific volume of the enzyme is assumed to be 0.735 milliliters per gram at 10° C, Archibald measurements indicate the molecular weight of urokinase to be 53,000.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0036-8075
1095-9203
DOI:10.1126/science.147.3660.880