Comprehensive landscape of the T and B-cell repertoires of newly diagnosed gestational diabetes mellitus

• Published in: Genomics (San Diego, Calif.) Vol. 115; no. 5; p. 110681
• Main Authors: Zhu, Hui, Zhao, Zhijia, Xu, Jin, Chen, Yanming, Cai, Jie, Shi, Chaoyi, Zhou, Liming, Zhu, Qiong, Ji, Lindan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2023
• Subjects: B cell receptor; Cytokines; Gestational diabetes mellitus; High-throughput sequencing; Immune repertoires; T cell receptor; T2DM; TCR; IgA; FH; ART; TRB; GTT; NT; Gestational diabetes mellitus; FBG; GDM; CDR3; BMI; PBS; AA; AC; T cell receptor; Cytokines; High-throughput sequencing; IR; IQR; IGH; EDTA; BCR; PCOS; MCH; HC; B cell receptor; OGTT; IADPSG; Immune repertoires
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2023.110681

This study conducted a high-throughput sequencing analysis of the T- and B- repertoires in the newly diagnosed GDM patients and evaluated the association between abnormal adaptive immunity and GDM. The unique TCR CDR3 clonotypes were mildly decreased in GDM patients, and the similarity of TCR V-J distributions was higher in the GDM group. Moreover, the usages of the V gene and V-J pair and the frequency distributions of some CDR3 amino acids (AAs) both in BCR and TCR were significantly different between groups. Moreover, the cytokines including IL-4, IL-6, IFN-γ and IL-17A were synchronously elevated in the GDM cases. Our findings provide a comprehensive view of BCR and TCR repertoires at newly diagnosed GDM patients, revealing the mild reduction in unique TCRB CDR3 sequences and slight alteration of the V gene, V-J combination and CDR3 (AA) usages of BCR and TCR. This work provides deep insight into the mechanism of maternal adaptive immunity in GDM and provides novel diagnostic biomarkers and potential immunotherapy targets for GDM. •Aberrant immune adaptation is involved in gestational diabetes mellitus (GDM).•Adaptive immune responses depend on the diversity of T- and B-cell receptors.•Total clonality of BCRs and TCRs were slightly reduced in GDM patients.•The usages of the V, J, V-J pair gene segments of BCR and TCR were changed with GDM.•The diversity of BCRs and TCRs were maintained in the GDM patients at onset stage.