Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria

• Published in: Food science and biotechnology Vol. 24; no. 2; pp. 427 - 431
• Main Authors: Kim, Min-Gi, Lee, Hoi-Seon
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.04.2015; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Clostridium difficile; Clostridium perfringens; Escherichia coli; Food Science; Nutrition; Research Article; 식품과학; intestinal bacteria; α-cedrene; growth inhibition; β-cedrene
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0056-5

The growth-inhibiting activities of the essential oils extracted from Platycladus orientalis leaves and stems were examined against intestinal bacteria. The leaf oil exhibited a strong response against Clostridium difficile and C. perfringens at 5.0 mg/disc, but the stem oil showed no inhibitory activity against intestinal bacteria. The oils from P. orientalis leaves and stems were analyzed by GC-Mass spectroscopy. The main constituents of the essential oil in P. orientalis leaves: α-pinene (18.5%), cedrene (13.6%), α-cedrol (11.3%), β-caryophyllene (10.2%), 3-carene (8.2%), and α-terpinyl acetate (5.1%), and in the stems: α-pinene (17.7%), 3-carene (16.3%), β-caryophyllene (13.4%), β-phellandrene (9.5%), β-selinene (6.5%), and α-cedrol (6.1%). The cedrene isomers showed significant (33.1–34.7 mm) inhibitory effect against C. difficile and C. perfringens and moderate inhibitory effect against Escherichia coli at 2.0 mg/disc. The oil of P. orientalis leaves, in which cedrene isomers are the major component, could be used as a selective inhibitor against harmful bacteria.