Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration

• Published in: Plant cell reports Vol. 22; no. 3; pp. 181 - 187
• Main Authors: GERMANA, M. A, CHIANCONE, B
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.10.2003; Springer Nature B.V
• Subjects: Androgenesis; Anther and ovule culture, utilization for haploid production; Biological and medical sciences; Biotechnology; Citrus - drug effects; Citrus - growth & development; Citrus - physiology; Eukaryotic cell cultures; Flowers - drug effects; Flowers - physiology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Phenylurea Compounds - pharmacology; Plant cells and fungal cells; Pollen - drug effects; Pollen - physiology; Seeds - physiology; Thiadiazoles; Culture medium; Anther; Pre-treatment; Citrus fruit; Citrus reticulata; Rutaceae; Tissue culture; Micropropagation; Androgenesis; Citrus; Haploidy; Regeneration; Improvement; Clementine; Dicotyledones; Haploid; Angiospermae; Plant growth substance; Ureas; Spermatophyta; Chemical composition; Phytohormone
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-003-0669-7

An improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4 degrees C, 25 degrees C, 32 degrees C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4 degrees C and 25 degrees C were more favorable for embryo production than 32 degrees C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time.