Germination rate of Phyllospadix japonicus seeds relative to storage methods and periods

• Published in: Ocean science journal Vol. 49; no. 1; pp. 67 - 72
• Main Authors: Park, Jung-Im, Lee, Kun-Seop, Son, Min Ho
• Format: Journal Article
• Language: English
• Published: Springer Korea Ocean Research and Development Institute and The Korean Society of Oceanography 01.03.2014; 한국해양과학기술원
• Subjects: Aquatic Pollution; Earth and Environmental Science; Earth Sciences; Marine; Marine & Freshwater Sciences; Oceanography; Phyllospadix; Waste Water Technology; Water Management; Water Pollution Control; 해양학; germination; habitat restoration; seed storage; recalcitrant seed
• ISSN: 1738-5261; 2005-7172
• DOI: 10.1007/s12601-014-0007-8

To determine the optimal storage method and longest possible storage period of Phyllospadix japonicus seeds, we examined post-storage germination rates using different storage methods and periods for P. japonicus seeds harvested in Korean coastal waters. P. japonicus seeds are classified as recalcitrant seeds with an average moisture content of 45.4%. Germination rates of P. japonicus seeds stored in seawater at 4 °C, seawater at room temperature with air supply, and an aquarium with continuous seawater circulation ranged from 35.0% to 43.5%, whereas seeds stored in seawater at 30°C, a refrigerator at −20°C, and a desiccator at room temperature did not germinate. Seeds stored at 4°C maintained germination rates of 72.5∼73.0% until 30 days of storage, but showed rapidly decreasing germination rates after 60 days and no germination after 180 days. Since few studies have investigated seed storage of P. japonicus , these results will serve as useful data for seed-based P. japonicus habitat restoration.