Effect of "external" superoxide anion on apoptosis in coleoptiles of wheat seedlings

• Published in: Biochemistry (Moscow) Vol. 70; no. 10; pp. 1095 - 1103
• Main Authors: Vorobjev, A A, Smirnova, E G, Bakeeva, L E, Yaguzhinsky, L S
• Format: Journal Article
• Language: English
• Published: United States Springer 01.10.2005; Springer Nature B.V
• Subjects: Anions; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; Cell Differentiation - drug effects; Cell Division - drug effects; Chromatin; Chromatin Assembly and Disassembly - physiology; Cotyledon - cytology; Cotyledon - drug effects; Dibutyl Phthalate - pharmacology; Mitochondria; Mitochondria - drug effects; Mitochondria - ultrastructure; Oxidation; Phthalic acid; Phthalic Acids - pharmacology; Seedlings; Seedlings - cytology; Seedlings - growth & development; Superoxide; Superoxide anions; Superoxides - pharmacology; Wheat
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1007/s10541-005-0230-0

A derivative of phthalic acid, dibutylphthalate (DBP), which has gametocidal effect at the concentration of approximately 10(-4) M, increased apoptosis in coleoptiles of wheat seedlings. This was associated with activation of chromatin margination and generation of mitochondria-containing vesicles. At the same concentration, DBP activated the release by the coleoptiles of superoxide anion into the environment. Lower (10(-5) M) and higher (10(-3) M) concentrations of DBP virtually had no effect on either process. A probable mechanism of effect of the "external" superoxide anion on apoptosis within the plant cell is discussed.