Functional Characterization of Salt‑Stress Induced Rare Cold Inducible Gene from Camelina sativa (CsRCI2D)

• Published in: Journal of plant biology = Singmul Hakhoe chi Vol. 65; no. 4; pp. 279 - 289
• Main Authors: Kim, Yeon-Ok, Kim, Hyun-Sung, Lim, Hyun-Gyu, Jang, Ha-Young, Kim, Eunsuk, Ahn, Sung-Ju
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.08.2022; Springer Nature B.V; 한국식물학회
• Subjects: Abiotic stress; Accumulation; Antioxidants; Biomedical and Life Sciences; Camelina; Enzymes; Flowers & plants; Gene expression; Genes; Germination; Growth conditions; Homeostasis; Hydrogen peroxide; Life Sciences; Localization; Membranes; Physiology; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant growth; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Proteins; Research Article; Salinity; Salinity tolerance; Salt; Salt tolerance; Seed germination; Seedlings; Seeds; Shoots; Tobacco; Yeast; 생물학; C-terminal hydrophilic tail; Salt tolerance; Reactive oxygen species; Rare-cold-inducible 2 protein; Antioxidant enzymes
• ISSN: 1226-9239; 1867-0725
• DOI: 10.1007/s12374-021-09313-6

Rare-cold-inducible 2 ( RCI2 ) genes are involved in plant response to abiotic stresses. In this study, we report the functional role of a Camelina RCI2, CsRCI2D, in plant salt stress response. The localization of CsRCI2D was observed in the plasma membrane and intracellular membranes by confocal analysis in tobacco leaf and western blot analysis in Camelina . The full length CsRCI2D cDNA clone was not able to complement the salt sensitivity of △spmp3 lacking the PMP3 gene. However, a C-terminal tail deleted CsRCI2D cDNA was able to restore the level of salt tolerance to that of WT. CsRCI2D -overexpressing Camelina showed better germination rate and seedling growth. CsRCI2D overexpression decreased Na + accumulation in both roots and shoots but increased K + accumulation in the shoots under salt stress. Furthermore, CsRCI2D -overexpressing Camelina displayed lower H 2 O 2 and malondialdehyde (MDA) upon salt stress. Under normal growth condition, CsRCI2D -overexpressing Camelina showed higher transcript levels of all antioxidant genes ( CsCuSODs, CsMnSOD1, CsFeSODs, CsCATs, CsAPX1, and CsGR ), whereas salt stress significantly induced all antioxidant genes in WT. These results indicate that salt-upregulated CsRCI2D plays a positive role in Camelina seed germination and seedling growth under salt stress by maintenance of ion homeostasis and modulating the expression of antioxidant-related genes.