Temporal expression of hippocampal lysophosphatidic acid receptors and their roles in kainic acid-induced neurotoxicity

• Published in: Genes & genomics Vol. 36; no. 2; pp. 239 - 246
• Main Authors: Lee, Jin-Koo, Kwon, Min-Soo, Kim, Hak Rim, Kim, Hyung-Gun, Sim, Yun-Beom, Park, Soo-Hyun, Suh, Hong-Won
• Format: Journal Article
• Language: English
• Published: Seoul The Genetics Society of Korea 01.04.2014; 한국유전학회
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Human Genetics; Life Sciences; Microbial Genetics and Genomics; Plant Genetics and Genomics; Research Article; 생물학; LPA receptor; Neuronal death; Lysophosphatidic acid; Kainic acid; Hippocampus
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-013-0162-9

In this investigation, the role of hippocampal lysophosphatidic acid (LPA) receptors in the regulation of kainic acid (KA)-induced neurotoxicity was investigated. KA (0.07 μg) intracerebroventricular (i.c.v.) administration increased hippocampal Lpar1, 2, 3, and 5 mRNA levels. In the immunohistochemical study, alteration of LPA 1 or LPA 3 immunoreactivity was different depending on the hippocampal regions, such as CA1, CA2, CA3, and dentate gyrus. In addition, the i.c.v. pretreatment with LPA 1 and LPA 3 antagonists, such as VPC12249 (0.05 μg) and VPC32183 (0.05 μg) attenuated KA-induced neuronal cell death in the hippocampal CA3 region. However, the i.c.v. 18:1 LPA (0.05 μg) pretreatment aggravated KA-induced neuronal cell death in the hippocampal CA3 region. Our results suggest that LPA receptors, such as LPA 1 and LPA 3 activation might play an important role in the regulation of KA-induced neuronal cell death in the hippocampal CA3 region.