Liquid-phase microextraction combined with high-performance liquid chromatography for the enantioselective analysis of mefloquine in plasma samples

A simple and rapid method, which involves liquid-phase microextraction (LPME) followed by HPLC analysis using Chiralpak AD column and UV detection, was developed for the enantioselective determination of mefloquine in plasma samples. Several factors that influence the efficiency of three-phase LPME...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 46; no. 5; pp. 929 - 936
Main Authors Magalhães, Igor Rafael dos Santos, Bonato, Pierina Sueli
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 14.04.2008
Subjects
Administration, Oral
Amylose - analogs & derivatives
Animals
Antimalarials - administration & dosage
Antimalarials - blood
Antimalarials - chemistry
Antimalarials - pharmacokinetics
Chemistry Techniques, Analytical - methods
Chemistry Techniques, Analytical - standards
Chromatography, High Pressure Liquid - standards
Hydrogen-Ion Concentration
Liquid chromatography
Liquid-phase microextraction
Male
Mefloquine - administration & dosage
Mefloquine - blood
Mefloquine - chemistry
Mefloquine - pharmacokinetics
Mefloquine enantiomers
Methanol - chemistry
Phenylcarbamates
Plasma samples
Rats
Rats, Wistar
Reproducibility of Results
Sodium Chloride - chemistry
Solvents - chemistry
Spectrophotometry, Ultraviolet
Stereoisomerism
Liquid-phase microextraction
Liquid chromatography
Plasma samples
Mefloquine enantiomers
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Summary:A simple and rapid method, which involves liquid-phase microextraction (LPME) followed by HPLC analysis using Chiralpak AD column and UV detection, was developed for the enantioselective determination of mefloquine in plasma samples. Several factors that influence the efficiency of three-phase LPME were investigated and optimized. Under the optimal extraction conditions, the mean recoveries were 33.2 and 35.0% for (−)-( SR-)-mefloquine and (+)-( RS)-mefloquine, respectively. The method was linear over 50–1500 ng/ml range. Within-day and between-day assay precision and accuracy were below 15% for both enantiomers at concentrations of 150, 600 and 1200 ng/ml. Furthermore, no racemization or degradation were seen with the method described.
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ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2007.01.043