A novel photometric glucose biosensor based on decolorizing of silver nanoparticles

• Published in: Sensors and actuators. B, Chemical Vol. 158; no. 1; pp. 185 - 189
• Main Authors: Tashkhourian, J., Hormozi-Nezhad, M.R., Khodaveisi, J., Dashti, R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.11.2011
• Subjects: Absorbance; Biosensor; biosensors; cost effectiveness; decolorization; detection limit; enzyme substrates; glass; Glucose; glucose oxidase; hydrogen peroxide; Localized surface plasmon resonance absorbance; nanosilver; Silver nanoparticles; silver nitrate; Sol–gel matrix; surface plasmon resonance; Localized surface plasmon resonance absorbance; Glucose; Sol–gel matrix; Biosensor; Silver nanoparticles
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2011.06.002

► A simplified glucose biosensor based on AgNPs decolorizing has been developed. ► SPR of AgNPs is a sensitive analytical signal for constructing glucose biosensor. ► Sol–gel as the immobilization matrix for constructing of glucose biosensor was used. A novel glucose biosensor based on chromophore (silver nanoparticles) decolorizing for the photometric determination of glucose was developed. Silver nanoparticles are directly synthesized in the sol–gel matrix by a one-step method based on the reduction of the inorganic precursor AgNO 3 and were used for the preparation, characterization and calibration of a highly sensitive and cost-effective localized surface plasmon resonance-based glucose biosensor. In the presence of glucose oxidase (GOx) and due to the enzyme–substrate (glucose) reaction, H 2O 2 was produced and silver nanoparticles in the sol–gel glass have the ability for the decomposition of hydrogen peroxide. Due to the degradation of silver nanoparticles a remarkable change in the localized surface plasmon resonance absorbance strength could be observed which have been monitored as a suitable signal for determination of substrate concentration. Beer's law is obeyed in the range from 50 to 800 mg/L glucose and the limit of detection is 23 mg/L. The proposed optical biosensor has been successfully applied to the determination of glucose in various real samples.