A simple, rapid, and sensitive method for measuring protein concentration in subcellular membrane fractions prepared by sucrose density ultracentrifugation

The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Suc...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 119; no. 2; pp. 424 - 427
Main Authors Simpson, Ian A., Sonne, Ole
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.01.1982
Subjects
adipocytes
Adipose Tissue - analysis
Animals
Centrifugation, Density Gradient - methods
Coloring Agents
density gradients
In Vitro Techniques
membrane proteins
Membranes - analysis
Proteins - analysis
Rats
Subcellular Fractions - analysis
ultracentrifugation
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Summary:The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Sucrose in concentrations up to 0.7 m interfere only moderately with the assay. A rapid and convenient method is, therefore, now available for multiple protein determinations following sucellular fractionation on sucrose gradients.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(82)90608-X