XPC Lys939Gln polymorphism, smoking and risk of sporadic colorectal cancer among Malaysians

• Published in: World journal of gastroenterology : WJG Vol. 19; no. 23; pp. 3623 - 3628
• Main Authors: Ahmad Aizat, Abdul Aziz, Siti Nurfatimah, Mohd Shahpudin, Aminudin, Mustapha Mohd, Ankathil, Ravindran
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 21.06.2013
• Subjects: Adult; Aged; Aged, 80 and over; Asian Continental Ancestry Group - genetics; Brief; Case-Control Studies; Chi-Square Distribution; Colorectal Neoplasms - ethnology; Colorectal Neoplasms - genetics; DNA-Binding Proteins - genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Heterozygote; Homozygote; Humans; Logistic Models; Malaysia - epidemiology; Male; Middle Aged; Odds Ratio; Phenotype; Polymorphism, Single Nucleotide; Risk Assessment; Risk Factors; Smoking - adverse effects; Smoking - ethnology; Malaysia; Cigarette smoking; Xeroderma pigmentosum group C Lys939Gln polymorphism; DNA repair; Susceptibility risk; Colorectal cancer
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v19.i23.3623

To investigate the risk association of xeroderma pigmentosum group C (XPC) Lys939Gln polymorphism alone and in combination with cigarette smoking on colorectal cancer (CRC) predisposition. Peripheral blood samples of 510 study subjects (255 CRC patients, 255 controls)were collected. DNA was extracted and genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. The association between polymorphic genotype and CRC predisposition was determined using the OR and 95%CI. The frequency of the homozygous variant (Gln/Gln) genotype was significantly higher in cases compared with controls (16.0% vs 10.2%, P = 0.049). The Gln/Gln genotype of XPC showed a significantly higher association with the risk of CRC (OR = 1.884; 95%CI: 1.082-3.277; P = 0.025). In the case of allele frequencies, variant allele C was associated with a significantly increased risk of CRC (OR = 1.375; 95%CI: 1.050-1.802; P = 0.020). Moreover, the risk was markedly higher for those who were carriers of the Gln/Gln variant genotype and were also cigarette smokers (OR = 3.409; 95%CI: 1.061-10.949; P = 0.032). The XPC Gln/Gln genotype alone and in combination with smoking increases the risk of CRC among Malaysians.