The NAD-linked α-glycerophosphate dehydrogenase of trypanosomes

NAD-linked α-glycerophosphate dehydrogenase plays a key role in the α-glycerophosphate cycle of Trypanosoma brucei . The activity in cell lysates was ample for this role. The enzyme was activated by salts (e.g. MgCl 2 or NaCl); it had a broad pH-optimum for the reduction of dihydroxyacetone phosphat...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 67; no. 2; pp. 538 - 543
Main Author Reynolds, C.H.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 17.11.1975
Subjects
Animals
Chlorides - pharmacology
Enzyme Activation
Glycerolphosphate Dehydrogenase - metabolism
Hydrogen-Ion Concentration
Kinetics
NAD
Species Specificity
Trypanosoma - enzymology
Trypanosoma brucei brucei - enzymology
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Summary:NAD-linked α-glycerophosphate dehydrogenase plays a key role in the α-glycerophosphate cycle of Trypanosoma brucei . The activity in cell lysates was ample for this role. The enzyme was activated by salts (e.g. MgCl 2 or NaCl); it had a broad pH-optimum for the reduction of dihydroxyacetone phosphate centred at pH 7.4, with an apparent K m of 0.5 mM; and it was weakly bound to particulate components of cell lysates. The enzyme from T. vivax was similar to that of T. brucei . These trypanosomal enzymes resemble that of the trypanosomatid Crithidia fasciculata , but are rather different from the enzymes of mammals, birds and insects.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(75)90845-1