Optimization of Aqueous Two Phase Extraction of Proteins from Litopenaeus Vannamei Waste by Response Surface Methodology Coupled Multi-Objective Genetic Algorithm

• Published in: Chemical product and process modeling Vol. 15; no. 1
• Main Authors: Saravana Pandian, P., Sindhanai Selvan, S., Subathira, A., Saravanan, S.
• Format: Journal Article
• Language: English
• Published: Berlin De Gruyter 01.03.2020; Walter de Gruyter GmbH
• Subjects: aqueous two phase system; Genetic algorithms; Ions; Molecular weight; multi-objective genetic algorithm; Optimization; Polyethylene glycol; Proteins; response surface methodology; Seafood; trisodium citrate; waste
• ISSN: 1934-2659; 1934-2659
• DOI: 10.1515/cppm-2019-0034

Waste generated from industrial processing of seafood is an enormous source of commercially valuable proteins. One among the underutilized seafood waste is shrimp waste, which primarily consists of head and carapace. is the widely cultivated shrimp in Asia and contributes to 90 % of aggregate shrimp production in the world. This work was focused on extraction as well as purification of value-added proteins from waste in a single step aqueous two phase system (ATPS). Polyethylene glycol (PEG) and trisodium citrate system were chosen for the ATPS owing to their adequate partitioning and less toxic nature. Response surface methodology (RSM) was implemented for the optimization of independent process variables such as PEG molecular weight (2000 to 6000), pH (6 to 8) and temperature (25 to 45 °C). The results obtained from RSM were further validated using a Multi-objective genetic algorithm (MGA). At the optimized condition of PEG molecular weight 2000, pH 8 and temperature 35 °C, maximum partition coefficient and protein yield were found to be 2.79 and 92.37 %, respectively. Thus, waste was proved to be rich in proteins, which could be processed industrially through cost-effective non-polluting ATPS extraction, and RSM coupled MGA could be a potential tool for such process optimization.