Draft genome sequence of Acinetobacter sp. neg1 capable of degrading ochratoxin A

• Published in: FEMS microbiology letters Vol. 362; no. 7
• Main Authors: Fanelli, Francesca, Chiara, Matteo, Liuzzi, Vania C., Haidukowski, Miriam, Tristezza, Mariana, Caterina, Manzari, D'Erchia, Anna Maria, Pesole, Graziano, Horner, David S., Mule', Giuseppina
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2015
• Subjects: Acinetobacter; Acinetobacter - genetics; Acinetobacter - metabolism; Base Sequence; Carcinogens; Contamination; Degradation; Food contamination; Food Microbiology; Genome, Bacterial; Genomes; Hydrolysis; Microbiology; Molecular Sequence Data; Mycotoxins; Nucleotide sequence; Ochratoxin A; Ochratoxins - metabolism; Phylogeny; Sequence Analysis, DNA; ochratoxin A; degradation; Acinetobacter
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnv004

Ochratoxin A (OTA) is a nephrotoxic and potentially carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium. It is one of the major mycotoxins contaminating grain, grapes and a variety of food products, and the development of methods for reducing pre- and post-harvest contamination has drawn considerable attention. In the current study, we isolated and sequenced the genome of a novel free-living Acinetobacter strain able to degrade OTA. Biochemical studies suggest that the degradation reaction proceeds via peptide bond hydrolysis. We report the genome sequence of an Acinetobacter strain capable of degrading ochratoxin A, a widely distributed contaminant of food products.