Langerhans cells in cutaneous tumours: immunohistochemistry study using a computer image analysis system

Immunohistochemistry, based on antibody anti-S100 protein, was used to evaluate the Langerhans cells (LC) in benign and malign skin neoplasias. These cells were quantitatively estimated using a computer image analysis (OPTIMAS software system, Version 6.1) in skin biopsies diagnosed as basal cell ca...

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Bibliographic Details
Published inJournal of molecular histology Vol. 37; no. 8-9; pp. 321 - 325
Main Authors De Melo, Jr, Mario Ribeiro, Araújo Filho, Jorge Luiz, Patu, Vasco José Ramos, Machado, Marcos Cezar Feitosa, Mello, Luciano Albuquerque, Carvalho, Jr, Luiz B
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.11.2006
Subjects
Aged
Basal cell carcinoma
Benign
Biopsy
Carcinoma, Basal Cell - pathology
Female
Humans
Image processing
Image Processing, Computer-Assisted - methods
Immunogenicity
Immunohistochemistry
Immunohistochemistry - methods
Keratosis
Langerhans cells
Langerhans Cells - pathology
Male
Medical diagnosis
Middle Aged
Morphometry
S100 protein
Skin cancer
Skin Neoplasms - pathology
Squamous cell carcinoma
Trichoepithelioma
Tumors
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Summary:Immunohistochemistry, based on antibody anti-S100 protein, was used to evaluate the Langerhans cells (LC) in benign and malign skin neoplasias. These cells were quantitatively estimated using a computer image analysis (OPTIMAS software system, Version 6.1) in skin biopsies diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborreic keratosis (SK) and actinic keratosis (AK). The antibody anti-S100 protein recognized them. No significant variations were observed in the number of LC among malignant tumour (BCC = 23.25 +/- 5.81 and EpC = 20.88 +/- 4.24). Benign lesions (AK = 33.04 +/- 7.11; TE = 55.74 +/- 9.35; SK = 42.38 +/- 9.92, and KA = 47.62 +/- 10.4) presented a higher number of LC when they were compared among them and to malignant and normal tissues. No significant differences were observed in LC area and volume between benign and malign neoplasias. These results indicate possibly differences in the immunogenicity between benign and malign epidermic tumours. In conclusion, the experimental computer assessment method was reliable and consistent to morphometric analysis of tumoural tissues.
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ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-006-9056-3