The effects of dicofol on induction of hepatic microsomal metabolism in rats

• Published in: Pesticide biochemistry and physiology Vol. 28; no. 3; pp. 362 - 370
• Main Authors: Narloch, Bruce A., Lawton, Michael P., Moody, David E., Hammock, Bruce D., Shull, Lee R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.1987; Elsevier
• Subjects: ACARICIDAS; ACARICIDE; ACARICIDES; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; BARBITURATES; BARBITURICOS; BARBITURIQUE; BETA-NAPHTHOFLAVONE; Biological and medical sciences; CYTOPLASMIC ORGANELLES; DDT; ENZIMAS; ENZYME; ENZYMES; ENZYMIC ACTIVITY; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; FOIE; HIGADO; INSECTICIDAS; INSECTICIDE; INSECTICIDES; LIVER; Medical sciences; METABOLISM; METABOLISME; METABOLISMO; ORGANITE CELLULAIRE; ORGANULOS CITOPLASMATICOS; Pesticides, fertilizers and other agrochemicals toxicology; RAT; RATA; RATS; Toxicology
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/0048-3575(87)90132-5

In a comparative study, the induction effects of dicofol, technical Kelthane, and DDT on hepatic microsomal and cytosolic enzyme activities in rats were compared with those effects produced by phenobarbital (PhB) and β-naphthoflavone (BNF). Male rats (ca. 250 g) were injected (ip) for 4 consecutive days with 1.0 ml of vehicle containing either dicofol (1.5, 15.0, 29.5, or 59.0 m M, Kelthane (dicofol content equal to 29.5 or 59.0 m M), DDT (59.0 m M), or BNF (36.7 m M). Liver weights, microsomal protein, and cytochrome P-450 concentrations and microsomal and cytosolic enzyme specific activities were measured. Dicofol produced dose-related increases in all of the parameters measured except liver weight and cytosolic epoxide hydrolase activity. At a concentration of 59.0 m M, dicofol increased the concentrations of microsomal protein (1.7-fold) and cytochrome P-450 (2.9-fold), and the specific activities of cytochrome c reductase (1.6-fold), ethoxycoumarin O-deethylase (2.3-fold), aminopyrine N-demethylase (3.0-fold), microsomal epoxide hydrolase (2.6-fold), and glutathione S-transferase (2.9-fold). The induction potency of dicofol was equivalent to Kelthane, DDT, and PhB at equimolar (59.0 m M) concentrations of chemical.