Detection Of Multiple Human Papillomavirus Types In Condylomata Acuminata From Immunosuppressed Patients
Using the hybrid capture method, condylomata acuminata from healthy patients (controls) and patients with altered cell-mediated immunity were analyzed for multiple human papillomavirus (HPV) DNA: 82.9% and 38.0% oflesions from 41 controls and 21 patients, respectively, were HPV DNA-positive only wit...
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Published in | The Journal of infectious diseases Vol. 170; no. 4; pp. 759 - 765 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
The University of Chicago Press
01.10.1994
University of Chicago Press |
Subjects | |
Online Access | Get full text |
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Summary: | Using the hybrid capture method, condylomata acuminata from healthy patients (controls) and patients with altered cell-mediated immunity were analyzed for multiple human papillomavirus (HPV) DNA: 82.9% and 38.0% oflesions from 41 controls and 21 patients, respectively, were HPV DNA-positive only with probes for low-risk HPV types (P = .00035). Using probes for both low- and high-risk HPV types, 16.3% and 52.3% of lesions from 43 controls and 21 patients, respectively, were positive for both probes (P = .0038). Evidence of multiple HPV types was also found by Southern blot and in situ hybridization studies. The mean HPV copy number detected by either probe did not differ significantly among patient groups. Using sensitive techniques, such as hybrid capture, multiple HPV types, including those associated with genital malignancy, can be detected in condylomata acuminata. Serial biopsies demonstrate the dynamic nature of genital HPV infection and that changes in the predominant HPV types may be reflected in tissue pathology. |
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Bibliography: | Reprints or correspondence: Dr. Darron R. Brown. Division of Infectious Diseases. Indiana University School of Medicine. Emerson Hall. Rm. 435. 545 Barnhill Dr.. Indianapolis. IN 46202–5124. ark:/67375/HXZ-JK8SGN4V-8 istex:43DCA06AC5E814DDF553C5FC20E732EC35826DDF ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0022-1899 1537-6613 |
DOI: | 10.1093/infdis/170.4.759 |