Hypobaric-hypoxia Induces Alteration in Microbes and Microbes-associated Enzyme Profile in Rat Colonic Samples

• Published in: Biomedical and environmental sciences Vol. 26; no. 10; pp. 869 - 873
• Main Authors: MAITY, Chiranjit, ADAK, Atanu, GHOSH, Kuntal, PATI, Bikas R, MONDAL, Keshab C
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013; Department of Microbiology, Vidyasagar University, Midnapore-721102, West Bengal, India%Special Center for Molecular Medicine, Jawaharlal Nehru University, New Mehrauli Road, New Delhi 110067, Delhi, India
• Subjects: Animals; Atmospheric Pressure; Bacteria, Aerobic; Feces; Hypoxia; Microbiota; Rats; 低压缺氧; 大鼠; 微生物相; 样品; 碱性磷酸酶活性; 结肠; 维修; 酶谱
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2013.013

Present study deals with the straight impact of hypobaric hypoxia on the quantity and composition of some predominant fecal microflora and its functional aspects. For that, isolated fecal contents of rat were exposed to two different simulated air pressures （70 kPa and 40 kPa） for different time durations （1, 3, and 5 h） and the bacterial community composition was compared with normobaric groups （101.3 kPa）. It was found that the total anaerobes, Escherichio coli, Enterbocters spp., Bi~idobocterium spp., CIostridium spp. were increased whereas total aerobes were decreased at both hypobaric treatments. The increased number of amplicon was detected in the pressure-treated groups than the control that clearly mentioned the disruption of microbiota structure at different simulated hypobaric-hypoxia. The amylase, protease, tannase, 13-glucuronidase, and alkaline phosphatase activities were increased at these atmospheric pressures. Thus, the present investigation demonstrates that the hypobaric hypoxia is an important environmental factor which can strongly modulate the composition of intestinal flora as well as microfiora-derived functional aspects.