Influence of chronic intrauterine hypoxia on development of testicles of newborns

• Published in: Russian open medical journal Vol. 7; no. 2; p. e0201
• Main Authors: Palatova, Tatiana V., Bucharskaya, Alla B., Medvedeva, Anna V., Voronina, Elena S., Maslyakova, Galina N.
• Format: Journal Article
• Language: English
• Published: Limited liability company «Science and Innovations» (Saratov) 01.01.2018
• Subjects: experiment; immunohistochemistry; intrauterine hypoxia; testes
• ISSN: 2304-3415; 2304-3415
• DOI: 10.15275/rusomj.2018.0201

Аim ― to assess the effect of chronic hypoxia on the development of the reproductive glands of the fetus of rats in experiment. Material and Methods ― In the work, 10 white outbred female rats aged 4 to 10 months with a weight of 200 ± 30 g were used. Laboratory animals were divided into 2 experimental groups, 5 rats in each. The first (experimental) group underwent hypoxia throughout the entire pregnancy (21 days). Modeling of hypoxia was carried out in accordance with the technique of N.N. Karkischenko (2010). The second (control) group was not exposed to any treatment throughout the entire pregnancy. Results ― There was a decrease in body weight in the offspring of the experimental group as compared to the control group.Histological examination of testicular tissue showed a significant decrease in the number of tubules in the field of vision, a decrease in the diameter and area of the tubules, with a simultaneous increase in the stroma area, a decrease in the proliferative potential, and an increase in the apoptosis of gonocytes, Leydig and Sertoli cells in the experimental group. Conclusion ― as a result of the conducted studies it was found that hypoxia in the antenatal period adversely affects the number and somatometric parameters of newborn rats in the offspring. Histological examination of testicular tissue showed a significant decrease in the number of tubules in the field of vision, a decrease in the diameter and area of the tubules, with a simultaneous increase in the stromal area, a decrease in the proliferative potential, and an increase in the apoptosis of gonocytes, Leydig and Sertoli cells in the test group rats. This indicates a delay and impaired tissue development testicles in conditions of hypoxia already in the antenatal period.